TASK: Write a maximum of 200 words each describing the phenotype of macrophages and osteoclasts – where are they derived from and what
is the functional role of each cell type? This will be handed in with your practical report
– the second question is TASK: Write a maximum of 200 words each describing M-CSF and RANKL –
what is the role of each cytokine, where are they derived from and
what is their effect on their target cell type?. This will be handed in with your practical report.
-Describe the functional role and the normal pattern of expression of:
1. GAPDH ,2. TRAP, 3 .c-Fm .
-These qusstions can be found in the prac manual that I will attach.
– The report should be set out with as follows: Abstract, Introduction, Aim, Methods (brief summary), Results and Discussion, Answers to the Practical Questions.
Abstract:
1- Short –single Paragraph.
2- Not the place to waffle on with background information
3- Aim (one sentence)
4- Method (overall Method) No details
5- Main result (one sentence about my result )
6- Overall conclusion (why doing practical) one sentence .
Introduction :
1- This provides the background to the experiments.
2- Sets the scene for why you carried out the experiment
3- What question are you trying to answer ?
4- Why is the topic important ?
5- What dose the reader need to know to understand you experiment ?
6- No method , no result
7- Finishes with a clear statement of aim (write the hypothesis as question) And the hypothesis (is that RANKL induce the tenparer expression of TRAP mRNA which were translated into TPAP protein leads to the differentiation of MQ into OSTEO’S.
Method : Write a very brief information, it is only one page for both experiments and refer to the practical manual and (Aurum Total RNA Mini Kit- please see the attached ) as references .
1- Practical stream 1 (Phenotypic changes during cellular differentiation.
2- Practical stream 2 (using gene expression changes to monitor macrophage and osteoclast differentiation.
Result : Present the data ( gel photo).
Discussion: You need to explain why you saw the results that you did Refer to literature . And write the Nile hypothesis (it is hypothesis that RANKL doesn’t induce the tenparer expression of TRAP mRNA which were translated into TPAP protein leads to the differentiation of MQ into OSTEO’S.
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